Key words: Listeria monocytogenes, poultry, processing plant, evisceration. ABSTRACT . Listeria antisera (Denka Seiken, Tokyo, Japan), accord- ing to the . Caracterização feno e genotípica de cepas de Listeria monocytogenes isoladas They were also serotyped (Denka Seiken, Japan), and sub–typed by PFGE. Antisera for the typing of Listeria Group O and H antigens. Aids public health and food testing laboratories in identifying sources of infection/contamination to.
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These strains were isolated from various sources, including produce, meats and dairy products, food processing environments, animals and humans, soils, and environmental samples. In order to help filling this gap, collecting some data from Brazil was one of the aims of this research. Cells were then washed, pelleted by centrifugation, and resuspended selken 0. In addition, we used antisera from the same serotyping kit in colony immunoblot experiments to identify mixed-serotype L.
Wells were washed twice with wash buffer 0. In general, strains of L. Academic Press, London, p.
After color development, alkaline phosphatase was inactivated by incubating the filters for 10 min in 50 mM EDTA, pH 8. Characterization of brazilian Listeria monocytogenes strains using DNA macrorestriction patterns.
Using the commercially prepared antiserum kit and dilution factors described, the ELISA protocol requires 0. Wiedmann for providing L. Isolates were confirmed as L. Open in a separate window.
The discrepancy was investigated by developing a serogroup-specific colony immunoblot method, which could distinguish these serogroups by differential staining Fig. Therefore, formaldehyde-treated cell suspensions and autoclaved cell suspensions were considered optimal for determining H-antigens and O-antigens, seikwn.
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Ecology of Listeria monocytogenes in the environment of raw poultry meat and raw pork meat processing plants.
This article has been cited by other articles in PMC. Filters were washed twice for 10 min each in wash buffer to remove cell debris and incubated in 20 ml of casein blocking solution for 1 h. Conversely, using a reference laboratory for serotyping large numbers of L.
Molecular subtyping to detect human listeriosis clusters. All other antimicrobial agents tested had no standardized breakpoints for Listeria spp. In addition, once listeriosis is not a disease under compulsory notification, there are no registers identifying L.
In order for these risks to be minimized, subtyping of L. The strains were identified in accordance with Rocourt et al.
In addition, formaldehyde-treated cells were prone to give false-positive reactions to O antiserum IX data not shown. Group I can be clearly divided in two clusters A and B with all the strains from cluster A belonging to serotype 4b. After digestion with ApaI and AscI, the strains were distributed in 3 different zeiken according to their profile.
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To esiken such scenario, public health laboratories in Brazil should become able to isolate and identify L. Considering the lack of information available in Brazil concerning listeriosis, the aim of this study was to analyze phenotypic and genotypically the L.
Acknowledgments We thank C. Serotyping of Listeria monocytogenes and related species. Serotyping was performed by a slide agglutination assay using commercially prepared antisera Listeria antiserum Seiken kit; Denka Seiken Co.
However, autoclaved cells tended to react less to negative O-factor antisera relative to cells treated by the other methods; that is, lower background reactions were observed. Colony lifts on nitrocellulose were probed sequentially using each O-factor antiserum as the primary antibody and a unique color alkaline phosphatase substrate to correspond to each primary antibody.
This method differentiates L. Muraoka for technical assistance; and J. Such strains would be indistinguishable from serotype 4b. Another source of variability in serotype identification may arise when L. Correlations between molecular subtyping and serotyping of Listeria monocytogenes.